Bacterial Transformation
Introduction
Biotechnology has to do with the manipulation of organisms to get useful products. One of the basis of biotechnology is genetic transformation. Genetic transformation occurs when DNA is taken in and expressed by a cell from a living organism. Three basic thing are needed to perform genetic transformation: a host, a vector, and a method to select and isolate the transformed organisms. The host is the organism that will take in the DNA. The vector is the means of transporting the DNA into the host. A common method for selecting the transformed organisms is to introduce them to an environment containing what they are resistant to (once they transform) because only the transformed organisms will be able to survive in that environment.
In this experiment, we will perform genetic transformation of the bacteria Escherichia coli (E. coli), therefore it is properly defined as bacterial transformation. E. coli will be our host. It is a good host for......
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