Enzymes lower the activation energy of chemical reactions but they themselves are not consumed or altered when doing so. These catalysts work best at optimum temperatures and pH’s. The temperature and pH at which the reaction occurs the quickest is the ideal condition for the enzymatic reaction.
Alpha amylase converts starch into glucose and when starch is combined with I2KI indicator a dark purple solution forms. As the enzyme breaks down the starch the absorbency will decrease. The absorbency is measured through the spectrophotometer which reads the transmittance of the wavelengths that pass through the solution. In order to determine the optimum temperature for the enzymatic reaction water bath of varying temperatures were made. To determine the ideal pH various buffers were added to the solutions in order to adjust the pH.
At scheduled intervals the reacting solution was added to a cuvette containing I2KI indicator in order to stop the reaction, allowing for accurate......
Join Now or Login to view the rest of this paper.
Approximate Word Count: 1958
Approximate Pages: 8 (260 words per double-spaced page) |