An experiment to demonstrate the effect of temperature on fermentation by yeast
The purpose of this laboratory is to observe how temperature affects the metabolism of
Grape juice by visibly noting the volume changes of identical food mixes containing yeast at different temperatures.
Background Information
If yeast is added to a liquid containing sugar and other nutrients, kept at an appropriate temperature (and deprived of oxygen), it will turn the sugars into ethanol (alcohol) and carbon dioxide.
Apparatus and Materials
Waterbaths set to a range of values - 10, 20, 30, 40, 50, 60, 70 °C
Thermometer
1 x 200 ml beaker - preferably marked on side
7 x test tubes
7 x 10 ml measuring cylinders
7 x Rubber bungs to fit the measuring cylinder & test tubes
Forceps
700 ml grape juice (Red)
70 ml yeast suspension
Plastic rod for mixing
Ice
Method
Step Method Justification
1 Prior to the experiment, make sure that the grape juice is shaken or mixed.
2 Set......
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