Factors Affecting The rate of Enzyme Activity
Prediction: As the temperature increases the rate of enzyme activity will also increase, thus increasing the rate of reaction. However, if the temperature is too high the enzyme will denature.
Materials: 4 test tubes
2 small beakers
A dozen filter paper disks
Test tube rack
Hydrogen peroxide (H2O2)
Potato extract
Forceps
Thermometer
Hot plate
Large beaker
Ice cubes
Graduated cylinder
Stopwatch
Procedure: Step 1 Place 10 mL of potato extract in a small beaker and check the temperature to ensure that it is at room temperature (20-25°C).
Step 2 Using forceps dip a filter paper disk into the extract and drop it into the test tube with 10mL of hydrogen peroxide.
Step 3 Allow the filter paper disk to sink to the bottom of the test tube (if the filter paper wont sink assist it to the bottom using a glass stirring rod). As soon as the Filter paper disk reaches the bottom begin the stop watch and stop the stopwatch when......
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