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Variation Of The Medium Composition And The Effects On Growth On Rhizopus Nigricans.


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ABSTRACT

Rhizopus nigricans was grown in various mediums with different carbon sources, nitrogen sources and at differing concentrations with all mediums adjusted to pH 5.5; growth was examined and recorded at the end of the experiment. Growth of the mycelial mass appears to caused by the Nitrogen Source, Corn Steep Liquor with it’s addition of miscellaneous trace metals and vitamins.

INTRODUCTION

Rhizopus nigricans is a type of mould that grows on bread and is commonly known as ‘Bread Mould’ (3). R. nigricans was subjected to varying types of carbon sources, Glucose monohydrate, Molasses, Starch and Lactose and at different concentrations, 2% and 4%. The different Nitrogen sources were Corn Steep Liquor 2.0%, Diammonium Hydrogen Phosphate (NH4)2HPO4 .2% , Urea .1%.

Variation of the media was done to investigate what affects the growth of R. nigricans.

MATERIALS AND METHODS

Mediums: 12 Mediums were prepared as shown in Table 1, labeled A-L with varying......

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Approximate Word Count: 1053
Approximate Pages: 5 (260 words per double-spaced page)

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