Introduction
The purpose to this lab was to isolate and identify two unknown bacteria from a mixed culture provided to us by our instructor. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Each test performed, provided us with some key information about the unknown microbes in question and how the bacteria function.
Materials and Methods
Over a two week period, eight prepared types of test media were provided to identify the assigned unknown mixed cultures. Not all of these tests were performed on every culture, as some were used only for gram positive or gram negative bacteria. The tests performed and what constituted a positive or negative test are as follows:
Lab day 1; today in lab we obtained the unknown mixed culture “041”and one brain-heart infusion agar (BHIA). The first step was the preparation of the medium, the bottom of the BHIA dish was labeled with the bacterium number,......
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