Making Molar Solutions and Dilutions
Objectives:
After performing this lab, the student should be able to:
• Calculate grams of solute and correctly prepare a molar solution.
• Prepare parallel and serial dilutions using C1V1 = C2V2
• Distinguish a parallel dilution from a serial dilution.
• Determine whether a parallel or serial dilution should be used in a given situation.
• Use a microcentrifuge to make a pellet.
Making Molar Solutions and Dilutions
A major job of any biotechnician is the preparation of solutions. Accuracy is of the utmost importance. An incorrectly
prepared solution can destroy months of hard work or cost companies thousands of dollars. Therefore, several safeguards
are in place in most SOPs (Standard Operating Procedures) to ensure that mistakes are minimized. First, all calculations are
written down in the lab notebook, even though a calculator is used to do the arithmetic. Second, important calculations are......
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