Discussion of Data and Results:
Chromatography serves as the most effective method to isolate and purify biomolecules. This experiment aimed to characterize proteins using the principle of chromatography, specifically, using gel filtration chromatography (a.k.a gel-exclusion chromatography). This method separates proteins and other macromolecules on the basis of size. This technique can be used particularly in molecular weight determination and on a quantitative analysis of their molecular interactions.
“All types of chromatographic methods are based on a very simple principle. The sample to be examined is allowed to interact with two physically distinct entities, a mobile phase and a stationary phase.” (Boyer, 2000) GFC exploits the differences at which molecules travel or pass through a bed of porous, semi-solid substance, which is the gel itself.
“The stationary phase consists of inert particles that contain small pores of a controlled size. A solution......
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