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- Investigation Of The Role Of MicroRNA- 223, MicroRNA -381 And MicroRNA- 505 In The Regulation Of Transcription Factor NFI-A.


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Introduction

It has been shown that the brain‘s tolerance to ischaemic injuries can be temporarily augmented when exposed to a non-injurious pre-conditioning stimulus (Gidday, 2006). The transcription factor NFI-A, a CCAAT-related binding protein that functions both in viral DNA replication and in the regulation of gene expression was found to be a gene that was upregulated during this phenomenon. It has also been shown to play a role in preconditioning-induced neuroprotective functions (Zheng et al, submitted).
Experiments have indicated that human NFIA is negatively controlled by miR-223, in that its translation is repressed by the binding of micro RNA 223 to a binding site on the 3’ UTR of the gene. (Fazi et al,2005).
MicroRNAs (miRNAs) are endogenously encoded, 22 nucleotide small noncoding RNAs that have been found to direct sequence specific down regulation by mRNA cleavage, cleavage-independent mRNA degradation or translational suppression of mRNA. (Pillai,2005 ;......

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Approximate Word Count: 1928
Approximate Pages: 8 (260 words per double-spaced page)

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