Bacteria Classification By Gram Staining
THE AMERICAN UNIVERSITY IN CAIRO
BIOLOGY DEPARTMENT
SCIENCE 453 : BIOLOGY FOR ENGINEERS REPORT No.1
Presented By : Karim A. Zaklama 92-1509 Sci. 453-01
24/2/96
Objective:
To test a sample of laboratory prepared bacteria and categorise it
according to Christian's gram positive and gram negative classes and also by
viewing it under a high powered microscope and oil immersions; classify its
shape and note any special characteristics.
Introduction:
Bacteria was categorised into two groups in 1884 by the Danish
Bacteriologist Christian, gram positive and gram negative by a staining
technique where the ability to avoid de-coloration of Crystal Violet solution
by alcohol would render the category of gram positive, and gram negative if the
bacteria is de-coloured. This could be noted by the final colour of the
bacteria: a violet colour where Gram positive and a pink colour of the Safranin
added pending the de-colouring process.
Materials:......
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