Title: Polymerase Chain Reaction Simulation
Propose:
The propose of this lab was to understand how by running a gel electrophoresis on a batch of DNA we are able to see how many approximately cycles it has gone through.
Methods: Casting the Agarose Gel In this experiment .8% solution was used. By using a 250ml flask the buffer solution was prepared. Using the equation to make enough solution for the entire lab class the equation had to be multiplied by four. The contents of this equation were added to the 250ml flask and swirled to evenly distribute it contents. Then a mark was placed on the outside of the flask to indicate the level of the solution before heating. The flask opening had perafilm placed over it so that there was little to no evaporation. The solution was then placed in the microwave and heated. The solution was then heated for one min and swirled for evenly dissolved Agarose. The Agarose was then cooled, so that it was not to hot and the plate would crack.......
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Approximate Word Count: 718
Approximate Pages: 3 (260 words per double-spaced page) |