I. Title
Identification of an Unknown Plasmid
In this experiment, we determined the phenotypic capability of an unknown plasmid along with its size. With the use of gel electrophoresis, we analyzed the gel photograph by using a standard DNA marker, Lambda HindIII, and came to a conclusion based on our results.
II. Abstract
Two experiments were done to identify an unknown plasmid. The success of these experiments came from the use of modern day technology involving gel electrophoresis. First, bacterial transformation to E. Coli DH5 was performed on our unknown plasmid along with two known plasmids, pAMP and pKAN, and a negative control TE, a buffer without DNA. By performing confluency streaking of bacteria in plates containing antibiotics, we were able to examine the recombinant DNA of the bacteria. After incubation of the plates, we analyzed the samples and found that our unknown plasmid reacted positively on the LB/AMP plate. There were a total growth of three......
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Approximate Pages: 14 (260 words per double-spaced page) |